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KMID : 1161520190230060407
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Animal Cells and Systems
2019 Volume.23 No. 6 p.407 ~ p.413
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AEG-1 aggravates inflammation via promoting NALP3 inflammasome formation in murine endometriosis lesions
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Zhao Juan
Ma Wei
Chen Weizhi
Gao Jie
Li Chunling
Tong Yahong
Zhou Qin
Zhao Xiuling
Wang Menghua
Xiao Huan
Jin Yanrong
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Abstract
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Endometriosis (EMs) is one of the most common gynaecological diseases in women of childbearing age. Astrocyte elevated gene-1 (AEG-1) is associated with the invasion, migration, apoptosis and prognosis of various cancers. However, the roles of AEG-1 in EMs and its corresponding molecular mechanism are still unknown. In this study, animal models of EMs were established and mice were divided into two groups (n?=?10): Sham group and EMs group. The EMs cells were isolated from EMs model. The AEG-1 gene was knocked down by shRNA, while the SOCS1 gene was knocked down by siRNA. Histological changes, AEG-1 expression in tissues and inflammatory factors level were detected by H&E staining, immunohistochemistry and ELISA, respectively. RT-qPCR and western blotting were used to determine the expression level of related proteins. The present study found AEG-1 was up-regulated in the EMs model. Enhanced AEG-1 promoted inflammatory cell infiltration, and elevated the levels of IL-1β, IL-6, and TNF-α in EM group (p?
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KEYWORD
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Endometriosis, astrocyte elevated gene-1, inflammation, nucleotide-binding oligomerization domain-like receptor 3, suppressor of cytokine signaling1
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